Parallel World Pharmacy

5: 6 Parent-Child Appraisal

"Whoa, you're gonna do it. Parent-child appraisal. This is gonna be a study, you're gonna see genomic information, right?

Palais came up with herself.

Pharma is in the process of establishing a biotechnology-based drug discovery and testing based on reagents brought back from different laboratories, but has not yet undertaken a paternity test. I guess I'm also interested in Palais on how to do the actual tests.

"Once, it can be done with hand-held equipment and reagents. Brother, that's why."

Pharma turns back to Palais.

"I'm going to get ready for the parent-child appraisal, but today I'm going to see some part-time pharmacists and training, and it's just me and Ellen. We don't have enough people, can you help the store?"

Then I'll help you.

Palais turned her thumb all the way to herself. Pharma is an obvious one, passing the medical records to Palais.

"Yeah, I'll do it alone."

Ellen glances.

"Ellen, it's tough on your own, brother, please."

Farma instructed Cedric to put a new "Primary Pharmacist Palais de Medicis" bill on the corner listing the pharmacist in charge for the day on the pharmacy wall. And give him a name tag to put on his chest. Ellen is the one who waited for it.

"Wait, Palle, can you handle the new drugs you have in this store? If I don't read the textbooks first, I'll have trouble getting a proper prescription for how much of a first-class medicine I am. Dosage is also dangerous if you make a mistake."

"I wrote that textbook, who do you think it is?

I say pallet abundantly disgusting.

"You're co-authored by me and my brother. Don't worry, Ellen, my brother's a rookie pharmacist, but it's okay if you ask him for medical attention or dispensation."

Pharma depresses the gavel. Ellen remembered that one of the authors of the pharmaceutical bible was Palle, and reluctantly pulled back.

"Oh, yeah. Ellen, can you get Sophie for me?

"Fine, but why?

Ellen shakes her neck.

"I need your help with something."

Farma explained that she didn't want to use this hand if possible, but had to ask Sofi for some experiments. Ellen did the use to the Bonufois and made them call Sophie.

And......

"You're late for practice, Eleonor. Practice and dispensing instead."

Complaints flew from the dispensary pallet as Pharma was preparing to and from the lab and dispensing room, and Ellen was practicing and requesting dispensing to Palle. Pallet wants to finish the patient quickly and see Pharma work.

"What? If you can do it that fast, do it."

Ellen raises her eyebrows tingly. Pallet took off his black coat and stepped out of the dispensing room in front of the patients. Palais's medical style is not white, but personal.

"I don't care how fast it is, you two be polite."

Pharma does not stop the work and complains from the dispensing room.

"Nah, naturally. How can you be grateful to see a first-degree pharmacist, civilians?

Patients accustomed to Pharma's lower back customer service are bewildered by the appearance and transverse attitude of unfamiliar youth pharmacists.

"What are you talking about all of a sudden, Palle? You have such a bad customer service attitude... sorry guys, I just got in today and I seem nervous"

"Oh? You got a complaint, Eleonor? Tables! Tables!

"Where I want it!

Pharma slackened as the two tried to get out onto the surface with their canes.

"What are you doing, both of you! If you leave the patient alone, I won't be ready."

Farma laments not having a brother with too much brain muscle.

"I'm sorry, my brother is being rude. Even this, I'm sure of my arm. I graduated from Novarut Medical University as chief, and I'm a little overconfident."

Pharma introduced the patients with an apology.

"The owner's brother."

"Then don't worry."

Reordered, Pallet mixes potion with patient saliva besides inspection, palpation, and auscultation, and combines speed practice with differential theology that discerns disease types with post-reaction potion color and precipitate, turbidity, smell, stickiness, etc., and the prescription of modern medicines.

"See you that fast, get to work... hey, Farma, that's okay"

"Ellen."

Pharma gives Ellen a note form in the dispensing room. See if Pharma's name matches the name of the patient he saw all the time and the name of the patient that Palle identified with divine arts.

"Wow... it fits"

Palle's diagnosis was perfectly consistent with Pharma's. And when I found out Palle was out of hand, something that the pills wouldn't cure, I didn't hesitate to throw it at Pharma. It means I know what I'm capable of and I can be objective.

"I regret it, but that's right, blood muscle. Where you are good at diagnostic skills"

Ellen is going to regret having only the master's son. The fact that Palle's patron saint is the medicine god also shapes the ability of pharmacists. Palais, and Bruno, when he is in the sanctuary of Pharma, he will receive protection, and his divine power and ability will rise. Fast diagnostic capability is one of them. When Pharma is on his side, he becomes more aware and more focused.

"Ellen does an accurate and polite job, so I'm helpful, though. Ellen is polite."

"Regardless of your attitude, Mr. Pallet, you can't go wrong fast. I'm a new pharmacist."

Ellen feels confident.

"Blood muscles are awesome"

It's not just the bloodstream.

Pharma knows that Palle is making a bloody effort, only in medicine and in divinity. Since Pharma cured leukemia, Palle began to think about the efficacy of modern medicine in its own right and to work more on pharmaceutical studies.

Ellen is also a hard worker, almost remembering her pharmaceutical textbooks, but she's either a genius skin.

"Oh, my God, call me here"

With a large number of squires, the Duke came to the pharmacy in a grumpy manner. After that, the Duchess comes in. Pharma rises and leaves the dispensing room to welcome the Duke.

"There you are, Duke. My name is Pharma, the court pharmacist, and I am the owner of this shop."

Meet with each other.

"At the request of your wife, we are going to conduct a parent-child appraisal between you, your wife and your child. The appraisal will be done fairly, and the witness will come?

"Mm... you, at the leave of His Holiness, are the chief medic of His Majesty the Emperor..."

This is the place, because I said so, or the Duke's words are badly toothed. On the occasion of the Argument Award, Pharma's name and face were well known, and Pharma was familiar.

Guide the line to and sit in a counseling booth divided by curtains.

"Parents and children, the appearance of which is only subjective. So let's find out what's going on with the blueprints of the human body."

"Blueprint?

"Rub the inside of your cheeks for two with this cotton swab. I don't have the strength. Witnesses are better off, baby."

Pharma put the cells of the oral mucosa taken with a cotton swab from the three parties on a slide glass with a clear name so that they could tell whose it belonged, set it on a microscopic stage and show the cells.

"There's something round, like a room"

"That's the cell (meaning the small room). The body of all animals is made up of each and every one of these cells. It's the smallest unit of organism."

"Hmm..."

"There's a part of that cell called the nucleus, and there's something inside that says chromosomes (chromosomes) that are dyed when immersed in dyes. Chromosomes are made of a substance called deoxyribonucleic acid, DNA."

Pharma undergoes a process of rupturing the cells to make them burst out in the nucleus of the cell, which took a while to process, so in the meantime Lotte sifts tea and sweets. It was getting awkward air. Pharma shows them the preparato.

"What... this, stringy thing, this stuff in my body"

"That's the chromosome."

Peeping into the microscope, the Duke looked at the purple-dyed, X-type chromosome and was wrapped around his tongue.

The lady looks anxious with the baby and watches over Yuki.

"Chromosomes are arranged in order of magnitude, and we call them in order of number, distinguishing them as if they were chromosomes."

There are 22 pairs of common sex chromosomes in humans, and you have two sex chromosomes that determine gender, Farma adds.

"I have 46 bottles in total..."

The Duke seemed to count to discipline.

"That's right. The blueprint of the human body engraved on this chromosome, that is, all genetic information, is called the genome."

"How do I read the information"

The Duke came on Pharma's pace, so Pharma gave him a grin hung on the dust and a play.

"Then join me in deciphering the genomic information."

So Pharma showed the textbook. Pharma textbooks with Lotte's illustrations are also useful for explaining.

"The genomic information of this chromosome is almost identical between individuals if they are human, but there are slightly different parts. Find out if a child has something that comes from a father, something that comes from a mother."

"What difference does it make?"

"Within the genome is a place called an iterative sequence, where the same cipher is repeated several to dozens of times. Find out how many times that cipher has been repeated."

So, Pharma takes out what he's been preparing to say.

"Here's an enzyme that replicates the genome."

Pharma shows a tiny tube at the tip of his finger, ice marinated in divine art.

It's an enzyme brought back from a different lab, a DNA synthetase. It is an enzyme that is fundamental to the technology of biotechnology.

"This enzyme is a worker who replicates the sequence of short nucleic acid fragments in two parts of the genome. Nucleic acid fragments are a small fraction of the genomic information, and we have several types available here."

Pharma shows tubes with DNA fragments called primers in modern times. I spoke fairly quickly about the explanation. I actually have a premise that I have to talk about a lot, but it's more challenging to talk to the average person.

"Enzymes, nucleic acid fragments, and genomes. By mixing these together and controlling the reaction temperature, DNA is exponentially amplified. This is called Polymerase Chain Reaction, PCR."

"Amplify it for what?

"Even if you can't see it in a single bottle, because if you have the amount, you can see the DNA, with the naked eye"

"With the naked eye!

The lady held her mouth, well.

"Now amplify the repetitive sequences that are characteristic of the individual by PCR method. But after that, let's see how many times. It will take a while to react, will you take a walk?"

Pharma had a PCR reaction over two hours. Since the pharmacy gatekeeper was a theological user of well-armed fire attributes, under Pharma's direction, he was asked to perform strict temperature regulation of heating and cooling on the sample to allow the reaction to proceed.

"Done. Here are sequences replicated only in specific regions by PCR reactions."

Pharma shows the tube with the end of the reaction. The Dukes came back for a light meal.

"How do I find out how many times"

The Dukes, who can't wait, seem to want to see results soon.

"Compare by length. Okay, here's the problem. Suppose you had a giant man and a little kid. If these two go through the crowd, which one will go through the crowd first?"

"It's a child. Giant guys run into people and slow down."

The lady snorts, too.

"Yes, then let's create a situation like that"

Sink the prepared rectangular cold weather into a strained solution in the bat and embed the post-reaction DNA solution on one side of the cold weather. Then, set the electrodes and wiring on the bat.

"Sophie, help me."

Pharma called Sophie and let her grip the electrode in both hands and flashed it with her favorite toy. Then, throughout the cold weather, electricity flows in one direction.

"In this cold weather it is reticulated and reproduces invisible sized crowds. And then, when you apply electricity from this end, the DNA is negatively charged, so -- pulled from the pole to the + pole, from the smaller size DNA to the goal in turn. It's called electrophoresis."

"Yeah? Yeah."

The Duke seemed a little confused since the time the electricity story came out, but several explanations seemed to swallow it. Sophie turned on the electricity and DNA separation began. DNA becomes banded and moves towards the goal in cold weather, Pharma says.

"I can't see anything moving, can I? Or I can't see the DNA."

In order to be visible to the naked eye, a PCR reaction was carried out, but the Duke wondered if he could see it.

"The DNA is clear, and it's not colored yet. Now we have a result, let's color the DNA so we can see it too, I wonder what's going on."

After 25 minutes, the DNA is stained so that it is visible to the naked eye. Pharma used staining reagents that showed color in visible light. Compare the results of the DNA of each of the father, mother, and child. Compare the distance the DNA travels. When dyed, banded lines were moving far from the area where the DNA was first implanted.

"This chunk of DNA, it's banded, so I call it a band, something that was pulled and moved by electricity by size. This is from the Duke, and this band belongs to his wife."

Pharma compares the travel distances of the bands of DNA in the three samples.

"Your child has both bands with the same distance traveled as these two bands. This time, it is separated by the size of the DNA, so if the distance traveled is the same, the DNA is the same size, that is, it has the same sequence of repetitions. There's about one in ten people with this pattern."

"Hmm..."

Both the Duke and the Lady can see that the DNA travel distances are consistent.

"Coincidental unanimity on one in ten, maybe, right?

Pharma hits the lead.

"So I've been looking into a few places this time. Right...... it all seems to match. So if you calculate the cumulative probability in this case, it's one in 30,000 people."

There are no 30,000 Imperial nobles.

"I mean, you're the only one in the capital."

Pharma sees the Duke's face. The Duke kept his mouth open.

"If you're not convinced, do you want more places to look?

Pharma turns a blind eye to Madame.

"Yes... no, no... I'm sorry. You... I'm sorry I said something terrible."

The Duke turned his doubts and heartless words to Her Ladyship. The lady was saving herself by the vivid proof of Pharma and was weeping.

"It's your child. No doubt about it, hold him."

My wife brings the baby closer to the Duke, who never tried to hold it.

"I think so too. Chances are, it's likely your child"

Farma, while avoiding certainty, told him to teach the Duke.

"Let's give him a name..."

The Duke lifted up my son with a tremendous gesture, but seemingly.

Drop off the three of you leaning in and leaving the pharmacy. The lady paid Pharma dearly. Pharma decided to gratefully receive it.

"Again, you owe me."

Pallet had completed all medical treatment and had also finished giving the medicine. Some aristocratic patients said they liked Palle and signed a contract to visit him as an attending pharmacist. Pharmatically, which has many patients. Thankfully, I don't care about patient embezzlement, but it's neat, Pharma laughed.

"Nevertheless, genes are honest."

Speak to Pharma like Ellen is relieved.

"Though not omnipresent. It's a record of life from ancient times."

That's what Pharma snorts too.

"I hope it works out, that family"

"I need you to come."

Genetic testing protected one family bond.