Plague Doctor

Cai Zixuan looked to his right and said: "Our team leader is Wang Ruoxiang. ”

“That's it?” Gu Jun frowned helplessly. What do you think it was? “It wasn't surprising. No, it wasn't her. I was surprised. ”

“Yeah, that's Wang Ruoxiang.” Cai Zixuan was very emotional.

Wang Ruoxiang is their squad leader, strong ability, beautiful person and good character. Such a schoolmaster goddess, of course, pursued her from the teaching building to the bureau of deconstruction. Tu Haojun was one of them. After that, it was called fierce, but eventually refused to end with Wang Ruoxiang's strict words: “I'm sorry, we're not one of a kind, I won't like people like you. ”

Uh, a tall, good-looking, wealthy, shitty, eight-year-old man?

At that time, everyone said thank you to Toho Jun and the class leader for making them believe in love again.

“The captain is still single.” Cai Zixuan obviously doesn't know how much of a bitch he looks right now.

“Don't look at me like that.” Gu Jun laughed at himself, "it's all over.” It's all over, Tu Haojun pursued the class leader a little liked it, and a little wanted to chase everyone's goddess, after all it had nothing to do with those profound things.

“Really?” Tsai Zixuan stopped talking about the master and looked at the watch. “It's almost 8: 00. I have to go to the lab. ”

“Come on, I'm going to the experimental building too.” Gu Jun said.

Tsai Zixuantong's eyes revealed a sense of mind. He just said that he didn't think about the sergeant. This turn is going to be followed by a lively crowd. It's really nice to be young.

“What?” Gu Jun was really helpless. He wanted to explain and didn't know where to start. “Forget it...”

“Yark Jun, you don't have to say, understand, there was a sea disaster for the water, I understand. ”

“You know a spongy body. ”

The Medical Experiment Building is in the northwest of the campus, a dozen floors of white-walled brick building with little exterior features, and even across the street from the doorway is an old piece of shitty cement. However, there are fully equipped laboratories in the building for the scientific research of teachers and students.

“This way.” After entering the building, Cai Zixuan walked forward and introduced Gu Jun as he walked.

The subject of their group's experiment, called "Studies on photodynamic treatment of tumors with several photosensitizers," was a study in which tumors were implanted subcutaneously in mice, then photodynamic therapy with different photosensitizers, and tumor changes were observed.

This split cost only 30,000 yuan, which is a lot for an undergraduate scientific research experiment, but an imported pure Photofrin photosensitizer costs tens of thousands of yuan. In order to be able to make several more photosensitizers, Wang Ruoxiang made a variety of fine calculations, saving money to re-establish the domestically produced imitation Photofrin group, dotiprofen group, ALA group, plus the blank control group, a total of 5 groups of mice.

“The research programme, the presentation of the questions were all taken care of by the team leader.” Cai Zixuan can't help but sigh when he says it, "You really deserve to be Wang Ruoxiang. ”

Although he is a mentor, Professor Ancient will only occasionally take the time to coach him. A big bull like his old man's is his business. So the Pioneer Cup still depends on the students themselves. The team leader does most of the work, but it's an opportunity that everyone can't ask for.

Because if the experiment produces a paper, Wang Ruoxiang is the first author, Cai Zixuan, they can only rank behind.

“We're still in the first phase, building a model of ascite-type neoplasm mice, which has been cultivated for a week, and today we can't see it. ”

Cai Zixuan said a great deal, suddenly found Gu Jun half silent, seemed to frown when he heard the word "tumor"...

“Uh, Yark Jun... you know, the success rate of modeling is not 100%. ”

Cai Zixuan scratched the bald spot before scratching his head. I really don't know what I just said. Is Gu Jun clear?

“What's 100 percent in the world?” Gu Jun lowered his head and covered his face, my friend, I haven't learned to do that yet, okay?

“True.” Cai Zixuan was very touched by the phrase, and his face burst with mulberry, “Everything worked out, like a dream bubble. ”

Okay, Gu Jun, I can't understand this...

During the conversation, the two men took the elevator to the eighth floor, where the laboratory used by their group was located.

Walking along the corridor, outside the lab door, Cai Zixuan pushed the door open and walked in first. When all the team members arrived, he became the last.

“Zixuan, wait for you. ”

“Huh?”

At this time, people saw a person walking in, and the lab stopped, leaving only the mouse chirping.

Tu Haojun...?

Didn't you say he dropped out of school and went around the world? I thought you said he was dead. Not him...

“Hello, everyone.” Gu Jun smiled and waved to everyone to greet Wang Ruoxiang, "Hello, Sergeant. ”

Wang Ruoxiang stood at the side of an experimental bench. Her short and medium hair was dark. Her delicate melon seed face was very beautiful, but her long body was covered in a white coat, and the whole person was very bright. Wang Ruoxiang saw him and frowned slightly. "Hello Gu Jun.” The sound is nice, too.

Gu Jun nodded at her for three months. The class leader was still so beautiful, but he was gone.

“Yark Jun was suspended from school before and is back now.” Cai Zixuan explained to everyone and introduced to Gu Jun: Xu Hai, a clinical classmate, is an acquaintance, and Zhang Hao Run, a basic medicine major, He Yuhan, a pharmaceutical major, a group of five people are present.

Cai Zixuan asked: "Sergeant, Yark Jun wants to take a look at us. Is it all right? ”

Wang Ruoxiang sighed silently and said: "Just stand aside and don't do anything. ”

Tu Haojun. She mumbled in her heart and remembered that she had a superficial fondness for him at the beginning of school a few years ago... vomit. This kind of person, delicious and lazy, full of poison, decaying wood can't be carved, rotten mud can't hold up against the wall... Rather than a mouse! It may only contribute to medicine if it is pulled away from the biopsy.

“It won't.” Gu Jun stood aside, feeling that Wang Ruoxiang's eyes were not good... illusion.

There was already a good row of mouse cages on the bench, each containing a group of white Kunming mice. This is the most commonly used experimental animal, very gentle and compact, weighs only 18-22g for 1-1.5 months and is less than 10cm long for experimental use.

Apart from a cage modeling mouse, all the others were blank mice, bought by Wang Ruoxiang from the school's experimental animal center. Once modeling is successful, they can be useful.

As the group arrived, everyone started working immediately.

Wang Ruoxiang wore rubber gloves and went to the modeling mouse cage to catch mice, all numbered by dyeing, and she caught mouse number 1. Cai Zixuan and several others are in charge of assistance, recording, video photography, etc.

“S180 mouse fibrosarcoma was implanted in the abdomen on Day 8.” Wang Ruoxiang's expression was focused and serious. He gently pressed the abdomen of mouse No. 1, which was obviously drummed up, and the mouse made a painful scream. She kept it in her hand.

“Mouse 1 has a growing ascites, is already a frog belly, has good vitality and does not need to be executed for the time being," she said. ”

From what she said, the crew were all a little excited at times, so they went over and looked at it clearly, "Is this the ‘frog belly’?” “Awesome. ”

“It's a frog belly, it's a big belly.” Cai Zixuan mourned.

Even though they're all eight years old, it's the first time they've done this kind of real scientific experiment.

“Oh.” Gu Jun jumped over to see what they were doing.

The reason why ascite-type tumor mice were first made was to obtain a large number of highly active S180 tumor cells. You know, the original cell lines are expensive, but one tube is enough to inject only one mouse, and the activity is fragile, and the four treatment groups they set up need to grow hundreds of mice.

So make it first, then expand it. Now the mouse has a frog belly, so it can "tuck in the belly.”

“Gu Jun.” Wang Ruoxiang suddenly turned his head and glanced at him, “Can you walk away? Obstructed. ”

“Yes.” Gu Jun shrugged his shoulder away. You can be angry. It can get in the way even a meter away from you.

Cai Zixuan, they all pretended not to see it, Wang Ruoxiang continued the next operation.

She pierced the abdominal cavity of mouse No. 1 with a 5ml syringe, successfully extracted a full tube of yellow clarified ascites and injected them into an Eppendorf undercone centrifuge tube, which she then took to centrifuge. Once centrifuged, a large amount of white precipitate appears at the bottom of the centrifuge tube, which is the tumor cell.

“Mouse 1 successfully modeled.” Wang Ruoxiang was relieved and smiled at the group members: “There are a lot of S180 tumor cells in the ascites! ”

“Yeah!" “Ha ha.” These are young people, nobody cares how mice feel. They cheer, "Tsai Zixuan praised.“ Take the first step to success! ”

The first step was successful, followed by subcutaneous tumors in mice treated with these cells, which were then photodynamic.

Much remains to be done, they continue to work, remove the supernatant from the centrifugal tube, resuspend the count, adjust the cell concentration...

To maintain cell activity, complete all injections within one hour. So they're busy modeling a mouse and then going to the next one.

“Adjust cell concentration to 1 × 10 ^ 7ml...”

“Yuhan, you are responsible for taking 0.2 ml of cells per needle. ”

“Captain, the narcotics are ready! ”

Every one of them was so ambitious, no one could care less about Tu Haojun, even forgot to stand by and watch him.

Gu Jun watched quietly, watched Ho Yuhan mix cell suspension, Cai Zixuan inject pentobarbital sodium into the abdominal cavity to anesthetize healthy mice, Xu Hai and Zhang Horan then numbered the mice and hair removal in the buttocks, and finally Wang Ruoxiang injected the mice right buttocks subcutaneously with tumor cells...

One, two, three, one after the other.

Suddenly, Gu Jun had a crack in his head. Is it a brain stem tumor attack...

The pain intensified, and he screamed, "Ah!” The scene in front of me became tranced and flickered like the shadow of a nightmare.

He seemed to be unable to breathe air, as if he was in a dilapidated lab, centrifuges, microscopes, sterilization pans and all kinds of other instruments had fallen to the ground, broken tubes, messy files, blurred frames, rotten animal bodies... dust turned all this into dusty colors, no breath of life, just chaos, death silence.

The pain in the brain was getting worse and clearer, and he saw some unspeakable instruments.

And those twisted bodies on the ground... can't even identify what animals are...

He also saw a line of blood on the inside wall, the kind of mysterious language on the medicine box, but compared to the printed body standard, it was a creepy weird line of blood.

Between the trances, Gu Jun suddenly seemed to understand the meaning of the line of blood.

He whispered.