Plague Doctor

Zhang Lin removes the cartridge from the liquid nitrogen tank, then removes a frozen storage tube from the cartridge, and carefully places the cartridge back because there are still many other cell freeze tubes in the cartridge. After Zhang Lin sealed the lid of the liquid nitrogen tank, a series of patient and meticulous operations began.

He first placed the frozen tube in a water bath pan, quickly thawed it with hot water at 37°C, then centrifuged the tube for five minutes, then sat down by the ultra-clean workbench, baked the centrifugal tube port to the alcohol lamp for disinfection, discarded the liquid, added fresh culture liquid with a pipette gun, repeatedly blew and mixed...

Gu Jun has been watching carefully beside him. If you have any questions, ask Brother Zhang for help. He has learned a lot.

These resuscitation operations, like centrifuge speed control, pipette gun absorption control, etc., have slightly so-so effects on cell survival and proliferation rates.

Finally, Zhang Lin injected the cell suspension into the dish, added the appropriate amount of culture solution, and placed it in a cell incubator at 37°C and 5% CO2.

“Wait, at least two days.” Zhang Lin took off his mask and pushed his glasses, "I suggest you keep it for a week, so that the tumorigenesis rate is guaranteed. ”

“Try one first for two days.” Gu Jun shrugged, "There's no way, there's not enough time. The system shows that he'll get worse in 30 days. If it's true, it might be difficult to walk out of bed by then. And he said," Brother, why don't you go out and rub it tonight? My treat. ”

“Forget it.” Zhang Lin waved his hand, “I also had to give the mouse medicine at night, which I bought. ”

……

In the next two days, Gu Jun spent time on campus. In addition to gathering together with friends, the first was to read books and study and prepare for the exam. If he could not die but had to repeat the grade, it would be quite troublesome; the second was to go to the experimental building every day to take care of the cells and change the culture liquid.

By the morning of the third day, Zhang Lin came to see for himself and said that the cells were barely grown enough. He helped pass on the culture and preparation of cell suspensions.

And Gu Jun went to the experimental animal center of the school, which is actually a large breeding base, a few hundred meters outside the school campus, very lonely, probably because it smells like various animals.

He bought five naked rats, over a hundred bucks each, pretty expensive, and fortunately he still has 20,000 bucks in his savings, but it won't last long.

When the money is used, I hate it very little. Gu Jun really regrets giving it away so early... people are still alive and the money is gone.

Cough. Each of these nude rats is two or three fingers in size, no hair, in a sterile box, and once exposed to a bacterial environment, they can easily get infected and die of disease. Therefore, routine feeding and experimentation processes need to be carried out in barrier environments.

The Eastern Barrier Environment Laboratory is at the Experimental Animal Research Center, a five-story yellow brick wall building next to the experimental building.

Gu Jun and Zhang Lin met in the lobby on the ground floor of the research center and booked a small bare mouse room for one hour. This is a small room of only 21 square meters, divided into a front room and a rear room with a laboratory table and instruments, and a rear room with a feeding room.

Before entering, they and all the items on the belt must undergo a strict ultraviolet sterilization. The bare mouse house was only stepped on by two people wearing special full-body protective clothing, masks, gloves, foot covers and other full gear.

Soon, at the anterior laboratory bench, Gu Jun labeled five nude mice by perforation and then injected them with tumor cells subcutaneously on the right side of one of their buttocks.

Zhang Lin was responsible for helping, and these cells hit people, and they could theoretically become tumors. Now that he sees Tu Haojun so hard on the naked rats, he is sure that this little brother is not trying to play murder.

After all five nude mice were injected, Gu Jun put them in the feeding cage and then saw if they could become tumors.

“Thank you, brother.” Gu Jun gave Brother Zhang a compliment. Without Brother Zhang's help, it would certainly not go so smoothly.

“The old TCM you said...” Zhang Lin still looks wary of Conan, "Aren't you really a liar? ”

……

In the following week, Gu Jun continued his previous rhythm of life, but went to the bare mouse room every day to see the bare mouse. Their feeding is under the unified care of the bare rathouse managers, who pay $3 per day for each meal and board.

He did only three general tasks this week, because those who needed medical help could not do so, and although the hospital was right next to him, he was not a doctor. The tasks he completed were related to some medical exercises, with five boxes of tablets in his hand, and Tyran's proficiency came first (30005000).

By the seventh day, Gu Jun again walked into the bare rat room in sterile gear and watched the bare rats one by one.

None of them died, and a yellow-bean-sized circular tumor had developed under the right side of the buttocks of No. 1, measuring 0.5 cm in diameter; protrusions were also evident on Nos. 3 and 4, 0.3 cm and 0.5 cm in diameter, respectively; and there were still no signs of tumorigenesis on Nos. 2 and 5.

So he can be sure, five nude rats, three tumors.

Gu Jun set two oncology nude mice as group A, one oncology nude mice as group B, two unoncology nude mice as group C and D, and went to the animal center to buy 10 healthy nude mice, 5 as group E, and 5 as group F. Groups A, C and E will receive the same daily dose of "Alien" (his name), while Groups B, D and F will not receive any doses for control purposes only.

In other words, adult nude mice, non-tumor nude mice and healthy nude mice, each have one group of medications, and one group does not use medications.

At this time, six cages had been divided on the test bench, and the naked rats were squeaking so loud.

Gu Jun tampered with 25 xenobiotic capsules. Since the instructions of the system are a box that can extend the life of 5 days, 5 capsules per box, that should be one capsule per day. This is an adult human dose, and these nude mice weigh less than 20 g on average, and if they also have one capsule a day, they are estimated to be sacrificed immediately.

He poured out all the powder in a capsule and weighed the dose, then converted it into a solution and diluted it proportionally.

Shortly after, Gu Jun grabbed a perfumed needle and grabbed a fixed healthy nude rat with his hand. He began the first gavage administration.

“Chirping.” The naked mouse struggled a little, but the poor thing, he pushed the needle and made its contribution to medicine.

At this moment, Gu Jun's heart beats a little faster and his eyes stare at the nude rat in his hand without blinking.

He had never tested the toxicity of an alien drug until this moment. If it dies suddenly, maybe the experiment loses its meaning. And now, he stares for five minutes, followed by 10 minutes...

Half an hour later, the nude mouse still showed no abnormality.

“It appears that there is no apparent acute toxicity to the allotropic drug.” Gu Junsong took a breath, "the chronic toxicity aspect needs to be observed. ”

He just kept filling the next nude rat. It took 10 minutes to complete the infusion of the other seven naked rats in the three dosing groups, and he observed for another hour that none of them had abnormal reactions, or were able to scream kinetic energy, drink water and eat feed.

“All you rat friends, let's live.” Gu Juncu mumbled, pressed the pain in the head, his condition is obviously getting worse, "please. ”

……

The next day and the third day, Gu Jun continued to come to the bare mouse room every day to work.

Tumors in undose group B nude mice had increased from 0.3cm to 0.6cm, and weight and mental status had not changed; while two naked mice in group A were given doses and came down for two days, the tumor size remained unchanged at 0.5cm.

Gu Jun knows what this could mean. Alien medicine really works! These capsules can significantly inhibit tumor growth.

And other doses of naked mice are as usual, which also means that alien drugs do not appear to have any adverse reactions and have little effect on physical functioning.

“Perhaps this time it's really saved?” He thought that while that anxiety was still haunting his mind, a new hope burned at the same time.

Day four, day five… a week goes by.

The tumour in group B nude mice has grown to 1.5 cm, and the large tumour contrasts sharply with its lean body, which begins to lose weight and become ill. While the two nude rats in group A continued to be stable, all naked rats administered were alive and well, similar to the nude rats in the blank control group.

I have to say, the results of this experiment are very satisfactory.

Alien drugs are better than the top oncology small molecule targeting drugs on the market today.

However, Gu Jun also knows that the adverse effects of Alien Medicine cannot be completely ruled out, which only proves that it has no side effects for a short time. And this is, after all, an animal experiment, which is somewhat different from a human clinical experiment.

But the results of this experiment are already worth the risk.

This afternoon, after writing his suicide note, Gu Jun came to the emergency department waiting area of Dongda Affiliated Hospital and sat down in a rare waiting bench seat. Surrounded by people and families, many crying children and consoling parents, nurses rushed around, caregivers pushed the rescue bed...

In a crowd, the broadcast of the emergency department kept calling: “182, Chen Ziyang. 183, Wang Yuxuan. 184, Huang Zixuan...”

After exhaling, Gu Jun resolutely threw an alien medicine capsule into his mouth and swallowed a sip of mineral water.

“Come on.” He looks down at the emergency signs on that wall, "if there's any toxicity, come on. ”